图2 五种喹诺酮类药物在鸡服药后270分钟后的色谱图
Figure 2 Chromatogram of plasma obtained from one chicken after the oral administration of five QNs in 270 min.
图 3 血药浓度与时间关系图
Figure 3 QNs plasma concentrations vs time curves in a representative subject. Plasma samples obtained upon oral administration of 20 mg of each of five QNs in capsule form to a 1.5 kg healthy female chicken.
表 5 五种喹诺酮类药物在鸡体内的药代动力学参数
Table 5 Pharmacokinetic parameters of five QNs in chicken after 20 mg single oral dose
Parameters
Units
DAN
LOM
GAT
FLU
OXA
t1/2(ka)
h
1.29
±0.07
1.52
±0.03
1.65
±0.05
1.47
±0.01
1.51
±0.02
t1/2(ke)
h
2.06
±0.16
1.78
±0.13
2.03
±0.25
1.69
±0.33
1.72
±0.16
C(max)
ng/mL
2701.98
±18.85
165.00
±23.05
1703.04
±31.14
1111.38
±21.00
895.75
±21.33
V/F(c)
kg
2.25
±0.31
3.14
±0.65
3.19
±0.46
4.72
±0.37
5.84
±0.49
AUC
(ng/mL).h
17641.70
±82.89
10886.89
±76.59
12260.74
±55.47
6869.67
±39.40
5661.45
±14.25
表 6 五种喹诺酮类药物在鸡血浆中的回收率
Table 6 Recoveries of five QNs spiked to plasma of one chicken after the oral administration of one capsule of five QNs in 270min (n=5)
Added
mg /mL
Found
LOM
GAT
OXA
FLU
DAN
mg/mL
mg/mL
mg/mL
mg/mL
mg/mL
0.50
Average
0.51
0.53
0.51
0.49
0.49
RSD(%)
1.0
2.5
2.4
1.5
2.5
Recovery(%)
103.00
105.40
101.20
98.00
98.00
5.0
Average
5.5
5.4
5.0
4.9
4.9
RSD(%)
1.1
1.8
2.5
2.4
3.0
Recovery(%)
110.00
108.00
100.00
98.00
98.00
25.0
Average
27.5
26.1
25.8
24.4
24.6
RSD(%)
2.6
4.8
3.4
2.0
4.3
Recovery(%)
110.00
104.40
103.20
97.60
98.40
4 结论
本研究建立一种采用反相高效液相色谱同时分离和测定生物体内五种喹诺酮类药物的方法。实验结果表明该方法简便、分析时间短,具有较高的灵敏度、精确度和重复性,可用于药代动力学及生物等效应的研究。
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